mcd99Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ balanced salt solution (HBSS). Cells were washed twice in phosphate-bufered saCharacterization of the interaction between mCD99 and mCD99L2. (A) Diagram of the DNA constructs for the expression of mCD99 or mCD99L2 proteins tagged with Myc, Flag, or HA